English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/58857
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Optimization of the pepsin digestion method for anisakis inspection in the fish industry

AuthorsLlarena-Reino, M. ; Piñeiro, Carmen ; Antonio, José Manuel ; Outeriño, Luis; Vello, Carlos; González, Ángel F. ; Pascual, Santiago
KeywordsAnisakids
CODEX STAN 244-2004
Digestion method
Fish
Liquid pepsin
Issue Date2013
PublisherElsevier
CitationVeterinary Parasitology 191(3-4): 276-283 (2013)
AbstractDuring the last 50 years human anisakiasis has been rising while parasites have increased their prevalence at determined fisheries becoming an emergent major public health problem. Although artificial enzymatic digestion procedure by CODEX (STAN 244-2004: standard for salted Atlantic herring and salted sprat) is the recommended protocol for anisakids inspection, no international agreement has been achieved in veterinary and scientific digestion protocols to regulate this growing source of biological hazard in fish products. The aim of this work was to optimize the current artificial digestion protocol by CODEX with the purpose of offering a faster, more useful and safer procedure for factories workers, than the current one for anisakids detection. To achieve these objectives, the existing pepsin chemicals and the conditions of the digestion method were evaluated and assayed in fresh and frozen samples, both in lean and fatty fish species. Results showed that the new digestion procedure considerably reduces the assay time, and it is more handy and efficient (the quantity of the resulting residue was considerably lower after less time) than the widely used CODEX procedure. In conclusion, the new digestion method herein proposed based on liquid pepsin format is an accurate reproducible and user-friendly off-site tool, that can be useful in the implementation of screening programs for the prevention of human anisakiasis (and associated gastroallergic disorders) due to the consumption of raw or undercooked contaminated seafood products
Description8 páginas, 2 figuras, 3 tablas
Publisher version (URL)http://dx.doi.org/10.1016/j.vetpar.2012.09.015
URIhttp://hdl.handle.net/10261/58857
DOI10.1016/j.vetpar.2012.09.015
ISSN0304-4017
E-ISSN1873-2550
Appears in Collections:(IIM) Artículos
Files in This Item:
File Description SizeFormat 
Optimization_pepsin_digestion.pdf263,51 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.