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Title

On-line monitoring of citric acid production in solid-state culture by respirometry

AuthorsPintado, José ; Lonsane, B. K.; Gaime-Perraud, I.; Roussos, S.
KeywordsSolid-state culture
Respirometry
Citric acid
Mussel processing wastes
Aspergillus niger
Issue Date1998
PublisherElsevier
CitationProcess Biochemistry 33(5): 513-518 (1998)
AbstractThe aim of this work was to study the possibility of monitoring citric acid production from mussel processing wastes (MPW) by Aspergillus niger in solid-state culture (SSC) on an inert support. This was conducted by measuring CO2 and 02 concentration in exhaust gases, using an automatic sampler connected to a gas chromatograph and a data acquisition system. The procedure permitted information on the physiological state of the culture to be obtained. A relationship between citric acid accumulation and a decreasc in CO, production was found, allowing citric acid production to be followed in real-time. Moreover, respiratory activity (p,.) can be estimated and the effect of different variables on this parameter studied. Initial nitrogen concentration, a critical factor for achieving high production of citric acid from MPW in submerged culture. revealed no effect in SSC. This indicates a tolerance of SSC to higher concentrations of nitrogen constituting an advantage when using residual media with high levels of protein and wlriability in their composition.
Description6 páginas, 4 figuras
Publisher version (URL)http://dx.doi.org/10.1016/S0032-9592(98)80060-5
URIhttp://hdl.handle.net/10261/58779
DOI10.1016/S0032-9592(98)80060-5
ISSN1359-5113
E-ISSN1873-3298
Appears in Collections:(IIM) Artículos
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