English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/58698
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Evaluating the in vitro metabolism of docosahexaenoic acid in sheep rumen fluid

AuthorsAldai, Noelia ; Hervás, Gonzalo ; Belenguer, Álvaro ; Frutos, Pilar ; Mantecón, Ángel R. ; Kramer, J. K. G.
KeywordsFatty acid metabolism
DHA
Biohydrogenation
GC
Metabolites
Incubation
Issue Date2012
PublisherSpringer
CitationLipids 47(8): 821-825 (2012)
AbstractRumen metabolism (e.g., biohydrogenation) of dietary unsaturated fatty acids (FA) is one of the main reasons why ruminant fats tend to be highly saturated and contain many isomerized FA intermediates. The process by which long-chain (20- to 24-carbon FA) polyunsaturated FA (LC-PUFA) are metabolized by rumen bacteria is not as well understood as that of linoleic or linolenic acids. In order to better understand the fate of LC-PUFA in the rumen several concentrations of docosahexaenoic acid (DHA) were evaluated in in vitro batch incubations ranging from 100 to 1,500 mu g per 6 mL of incubation volume using rumen fluid from sheep and incubated for 0, 1, 2, 3, and 6 h. From the results, it was shown that DHA was extensively metabolized at low (100 to 300 mu g/6 mL incubation volume), but not at high level of inclusion (800 mu g). At 300 mu g of DHA most of the depleted DHA was recovered as LC-DHA metabolites within the first 6 h of incubation, and at the lowest levels (100 mu g of incubation volume) further metabolism is apparent at 6 h. Using SP-2560 GC columns several LC-DHA metabolites were shown to elute after 24:0 and just past DHA, a region generally free of interfering FA. The present in vitro study would appear to be a useful method to evaluate the production of DHA metabolites in combination with its depletion.
Description5 páginas, 2 figuras.-- The original publication is available at www.springerlink.com
Publisher version (URL)http://dx.doi.org/10.1007/s11745-012-3688-8
URIhttp://hdl.handle.net/10261/58698
DOI10.1007/s11745-012-3688-8
ISSN0024-4201
Appears in Collections:(IGM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.