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http://hdl.handle.net/10261/58685
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dc.contributor.author | Trubetskoj, O. A. | - |
dc.contributor.author | Trubetskaya, O. E. | - |
dc.contributor.author | Afanas'eva, G. V. | - |
dc.contributor.author | Reznikova, O. I. | - |
dc.contributor.author | Sáiz-Jiménez, Cesáreo | - |
dc.date.accessioned | 2012-10-23T11:26:37Z | - |
dc.date.available | 2012-10-23T11:26:37Z | - |
dc.date.issued | 1997 | - |
dc.identifier | doi: 10.1016/S0021-9673(97)00019-8 | - |
dc.identifier | issn: 0021-9673 | - |
dc.identifier | e-issn: 1873-3778 | - |
dc.identifier.citation | Journal of Chromatography A 767(1-2): 285- 292 (1997) | - |
dc.identifier.uri | http://hdl.handle.net/10261/58685 | - |
dc.description.abstract | A humic acid (HA) from chernozem soil has been fractionated either by size-exclusion chromatography (SEC) on Sephadex G-75 using water, Tris-HCl or 7 M urea as eluents, or by ultrafiltration (UF) in the presence of 7 M urea or water. UF-fractionated HA was classified as 100K (nominal retention size >100 000); 30K (100 000-30 000); 10K (30 000-10 000); 5K (10 000-5000). Several pools from each chromatography and all ultrafiltration retentates were assayed by polyacrylamide gel electrophoresis (PAGE). The results indicate that SEC fractionation gave a better separation of HA on fractions differing in electrophoretic mobility and molecular size (MS), and that apparently SEC is a more feasible technique than UF for soil HA fractionation; nevertheless the UF in 7 M urea solution might be useful for MS evaluation of HA fractions obtained by association SEC-PAGE. PAGE in the presence of denaturing agents can be successfully used for checking the purity of HA fractions obtained by both SEC and UF. | - |
dc.description.sponsorship | The research described in this paper was made possible in part by grant MU000 from the Interna- tional Science Foundation (ISF) and cooperative grant M U 0 3 0 0 between the Russian Government and ISF. The authors also benefitted from the Cooperation Agreement between the Russian Academy of Sciences and the C.S.I.C. and a grant from the Junta de Andalucía for scientific exchanges. | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.rights | closedAccess | - |
dc.title | Polyacrylamide gel electrophoresis of soil humic acid fractionated by size-exclusion chromatography and ultrafiltration | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/S0021-9673(97)00019-8 | - |
dc.date.updated | 2012-10-23T11:26:37Z | - |
dc.description.version | Peer Reviewed | - |
dc.contributor.funder | Junta de Andalucía | - |
dc.contributor.funder | Russian Academy of Sciences | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100002674 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011011 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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