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Title

Polyacrylamide gel electrophoresis of humic and fulvic acids after acid hydrolysis

AuthorsTrubetskaya, O. E.; Trubetskoj, O. A.; Sáiz-Jiménez, Cesáreo
KeywordsFulvic acids
Humic acids
Acid hydrolysis
Polyacrylamide gel electrophoresis
Molecular size distribution
Issue DateJul-2001
PublisherTechnische Universität München
CitationFresenius Environmental Bulletin 10(7): 635-637 (2001)
AbstractTwo soil humic acids (HA), a marine sediment HA, and a soil fulvic acid (FA), were fractionated by polyacrylamide gel electrophoresis in the presence of denaturing agents before and after 6N HCl hydrolysis. After acid hydrolysis, the intensity of the high molecular size (MS) fraction decreased considerably in all HAs. On the other hand, a new high-MS fraction appeared in the FA after hydrolysis. The electrophoretic data indicate that acid hydrolysis produced a transformation in the humic macromolecule, inducing either depolymerization or condensation, depending on the nature of the humic fraction. Therefore, the advantages and disadvantages of using acid hydrolysis should be carefully considered before this treatment is carried out.
URIhttp://hdl.handle.net/10261/58676
Identifiersissn: 1018-4619
Appears in Collections:(IRNAS) Artículos
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