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Comparison of different methods to produce single-strand DNA for identification of canned tuna by single-strand conformation polymorphism analysis

AuthorsRehbein, Hartmut; Mackie, I. M.; Pryde, Susan E.; González Sotelo, Carmen ; Pérez Martín, Ricardo Isaac ; Quinteiro, Javier; Rey Méndez, Manuel
KeywordsCanned tuna
Polymerase chain reaction
Single-strand conformation polymorphism
Issue Date1998
CitationElectrophoresis 19(8-9): 1381-1384 (1998)
AbstractBy using single-strand conformation polymorphism (SSCP) analysis of three amplicons of the cytochrome b gene obtained by the polymerase chain reaction (PCR) it was possible to differentiate between various species of tunas and bonitos processed as canned fish. Four different techniques were used to produce single-strand DNA (ssDNA): (i) Denaturation of double-strand DNA (dsDNA) by formamide and alkali, (ii) two-step asymmetrical PCR, (iii) one-step asymmetrical PCR, and (iv) exonuclease digestion of the phosphorylated strand of dsDNA. The technique rendering optimal results depended on the type of amplicon (i.e. the sequence).
Description4 páginas, 5 figuras
Publisher version (URL)http://dx.doi.org/10.1002/elps.1150190830
Appears in Collections:(IIM) Artículos
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