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dc.contributor.authorÁlvarez-Alfageme, Fernando-
dc.contributor.authorFerry, Natalie-
dc.contributor.authorCastañera, Pedro-
dc.contributor.authorOrtego, Félix-
dc.contributor.authorGatehouse, Angharad M.R.-
dc.identifier.citationTransgenic Research 17(5):943-954(2008)es_ES
dc.description12 páginas, 6 figuras, 2 tablas -- PAGS nros. 943-954es_ES
dc.description.abstractThe present study investigated prey-mediated effects of two maize varieties expressing a truncated Cry1Ab, Compa CB (event Bt176) and DKC7565 (event MON810), on the biology of the ladybird Stethorus punctillum. Although immuno-assays demonstrated the presence of Cry1Ab in both prey and predator collected from commercial maize-growing fields, neither transgenic variety had any negative effects on survival of the predator, nor on the developmental time through to adulthood. Furthermore, no subsequent effects on ladybird fecundity were observed. As a prerequisite to studying the interaction of ladybird proteases with Cry1Ab, proteases were characterised using a range of natural and synthetic substrates with diagnostic inhibitors. These results demonstrated that this predator utilises both serine and cysteine proteases for digestion. In vitro studies demonstrated that T. urticae were not able to process or hydrolyze Cry1Ab, suggesting that the toxin passes through the prey to the third trophic level undegraded, thus presumably retaining its insecticidal properties. In contrast, S. punctillum was able to activate the 130 kDa protoxin into the 65 kDa fragment; a fragment of similar size was also obtained with bovine trypsin, which is known to cleave the protoxin to the active form. Thus, despite a potential hazard to the ladybird of Bt-expressing maize (since the predator was both exposed to, and able to proteolytically cleave the toxin, at least in vitro), no deleterious effects were observedes_ES
dc.description.sponsorshipThis work was supported by a grant from the Spanish Ministry of Environment. F. Álvarez-Alfageme was a recipient of a fellowship from the Comunidad de Madrides_ES
dc.subjectNon-target arthropodses_ES
dc.subjectBt maizees_ES
dc.subjectDigestive proteaseses_ES
dc.subjectLadybird Stethorus punctillumes_ES
dc.subjectTetranychid mites Tetranychus urticaees_ES
dc.titlePrey mediated effects of Bt maize on fitness and digestive physiology of the red spider mite predator Stethorus punctillum Weise (Coleoptera : Coccinellidae)es_ES
dc.description.peerreviewedPeer reviewedes_ES
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