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Título: | Species identification of cooked fish by urea isoelectric focusing and sodium dodecylsulfate polyacrylamide gel electrophoresis: A collaborative study |
Autor: | Rehbein, Hartmut; Piñeiro, Carmen CSIC ORCID; Pérez Martín, Ricardo Isaac CSIC ORCID | Fecha de publicación: | 1999 | Editor: | Elsevier | Citación: | Food Chemistry 67(4): 333-339 (1999) | Resumen: | The suitability and reliability of urea IEF and SDS-PAGE for the identi®cation of cooked ®sh ¯esh was tested by a collaborative study among nine laboratories. Urea IEF was performed with CleanGels as well as with ImmobilineGels, and ExcelGels were used for SDS-PAGE, enabling all three types of gels to be run in the same ¯at bed electrophoresis chamber. By strictly following opti- mised standard operation procedures (SOPs), ®ve unknown cooked samples had to be identi®ed with each technique using a set of 10 raw reference samples. With urea IEF, only one out of 35 identi®cations was incorrect, and with SDS-PAGE a similar result was obtained. It was concluded that methods, as now developed, are suitable for checking the species declaration of ®shery products. | Descripción: | 7 páginas, 3 tablas, 3 figuras.-- Hartmut Rehbein ... et al. | Versión del editor: | http://dx.doi.org/10.1016/S0308-8146(99)00175-2 | URI: | http://hdl.handle.net/10261/57504 | DOI: | 10.1016/S0308-8146(99)00175-2 | ISSN: | 0308-8146 |
Aparece en las colecciones: | (IIM) Artículos |
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Species_identi_cooked_fish.pdf | 229,09 kB | Adobe PDF | Visualizar/Abrir |
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