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Fish species identification in canned tuna by PCR-SSCP: Validation by a collaborative study and investigation of intra-species variability of the DNA-patterns

AuthorsRehbein, Hartmut; Mackie, I. M.; Pryde, Susan E.; González Sotelo, Carmen ; Medina, Isabel ; Pérez Martín, Ricardo Isaac ; Quinteiro, Javier; Rey Méndez, Manuel
KeywordsFish species identification
Canned tuna
Issue Date1999
CitationFood Chemistry 64(2): 263-268 (1999)
AbstractAnalysis of single strand conformation polymorphism (SSCP) of an amplicon (123 bp) obtained by polymerase chain reaction (PCR) of the mitochondrial cytochrome b gene was used to identify the ®sh species in canned tuna. Single-stranded DNA (ssDNA) was separated by polyacrylamide gel electrophoresis, and visualised by silver staining. The reliability of the method was tested by a collaborative study in which eight European laboratories participated. Seven unknown samples (®ve from individual species and two mixtures of two tuna species) of canned tuna had to be identi®ed by comparison with reference material. From a total of 72 cases, 65 (90.3%) were assigned correctly. Intra-species variability of SSCIP patterns was found in the case of Katsuwonus pelamis and Sarda sarda. As specimens from various ®shing grounds gave two or three di erent patterns of ssDNA, the possibility of some variability of the DNA patterns has to be considered in SSCP analysis of these species
Description6 páginas, 4 figuras, 2 tablas
Publisher version (URL)http://dx.doi.org/10.1016/S0308-8146(98)00125-3
Appears in Collections:(IIM) Artículos
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