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NapA and NapB are the Aspergillus nidulans Nap/SET family members and NapB is a nuclear protein specifically interacting with importin alpha

AuthorsAraújo-Bazán, Lidia CSIC ORCID ; Fernández-Martínez, Javier; Ríos, Vivian de los CSIC ORCID ; Etxebeste, Oier; Albar, Juan Pablo; Peñalva, Miguel Ángel CSIC ORCID ; Espeso, Eduardo A. CSIC ORCID
KeywordsAspergillus nidulans
Importin α
Nucleosome assembly proteins
Nuclear transport
Issue DateMar-2008
CitationFungal Genetics and Biology 45(3):278-291(2008)
AbstractIn eukaryotic cells, importin α is the major carrier for transport protein cargoes into the nucleus. We characterize here kapA, the single Aspergillus nidulans gene encoding an importin α. Using an affinity approach, we identify six potential interactors of KapA50, a deleted version of KapA lacking the autoinhibitory importin-beta-binding domain. One such interactor is NapB, the A. nidulans orthologue of Saccharomyces cerevisiae Vps75p, a histone chaperone member of the Nap/SET family of proteins that additionally plays a cytosolic role in vacuolar protein sorting. NapB, but not its close relative NapA (the A. nidulans orthologue of yeast Nap1p) interacts directly with KapA50 in pull down assays, despite the fact that NapB does not contain a classical nuclear localization sequence. NapB is a nuclear protein which exits nuclei at the onset of mitosis when two simultaneous mechanisms might be acting, the partial disassembly of the nuclear pore complexes and as yet unidentified posttranslational modification of NapB. The mitotic cytosolic localization of NapB might facilitate its putative role in the sorting of protein cargoes to the vacuole. In addition, we show that NapB and the mitotic B-type cyclin NimE compete for in vitro binding to KapA
Description14 páginas, 7 figuras, 4 figuras suplementarias, 1 tabla, 1 tabla suplementaria -- PAGS nros. 278-291
Publisher version (URL)http://dx.doi.org/10.1016/j.fgb.2007.08.003
Appears in Collections:(CIB) Artículos
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