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dc.contributor.authorLópez-Montero, Iván-
dc.contributor.authorArriaga, Laura R.-
dc.contributor.authorMonroy, Francisco-
dc.contributor.authorRivas, Germán-
dc.contributor.authorTarazona, Pedro-
dc.contributor.authorVélez, Marisela-
dc.date.accessioned2012-10-04T10:51:04Z-
dc.date.available2012-10-04T10:51:04Z-
dc.date.issued2008-04-15-
dc.identifier.citationLangmuir 24(8): 4065-4076(2008)es_ES
dc.identifier.issn0743-7463-
dc.identifier.urihttp://hdl.handle.net/10261/57296-
dc.description12 páginas, 8 figuras -- PAGS nros. 4065-4076es_ES
dc.description.abstractWe have studied the equilibrium and linear mechanical properties of model membranes of Escherichia coli built up as Langmuir monolayers of a native lipid extract using surface thermodynamics, fluorescence microscopy, and surface rheology measurements. The experimental study has been carried out at different temperatures across the physiological operative range 15−37 °C. Lipid phase coexistence has been revealed over a broad pressure range by fluorescence microscopy. The presence of ordered domains has been invoked to explain the emergence of shear elasticity accompanying the hydrostatic compression elasticity typical of fluid monolayers. The surface rheology measurements point out the soft character of E. coli membranes; i.e., upon deformation they react as a near-ideal compliant body with minimal energy dissipation, thus optimizing the effectiveness of external stresses in producing membrane deformations. These mechanical features appear to be independent of temperature, suggesting the existence of a passive thermoregulation mechanismes_ES
dc.description.sponsorshipThis work was financed by MEC under grants FIS2006-01305 and BFU2005-04087-C02 and by CAM S-0505/MAT/0283es_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.rightsclosedAccesses_ES
dc.titleHigh fluidity and flexibility of the inner membrane of Escherichia coli revealed by surface rheology of model Langmuir monolayerses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1021/la703350s-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1021/la703350ses_ES
dc.identifier.e-issn1520-5827-
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