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dc.contributor.authorPiñeiro, Carmen-
dc.contributor.authorBarros-Velázquez, J.-
dc.contributor.authorPérez Martín, Ricardo Isaac-
dc.contributor.authorGallardo, José Manuel-
dc.date.accessioned2012-10-02T08:14:53Z-
dc.date.available2012-10-02T08:14:53Z-
dc.date.issued2000-
dc.identifier.citationFood Chemistry 70(2): 241-245 (2000)es_ES
dc.identifier.issn0308-8146-
dc.identifier.urihttp://hdl.handle.net/10261/57041-
dc.description5 páginas, 4 figurases_ES
dc.description.abstractThe presence of specific enzymes in the sarcoplasmic protein fraction of eight gadoid fish species has been investigated following isoelectric focusing (IEF) in the 3.5–9.5 pH range. Lactate dehydrogenase (LDH) and glycerol-3-phosphate-dehydrogenase (G-3-PD) allowed the differentiation of most of the gadoids tested, even in the case of closely related species belonging to the genus Merluccius. Investigation of arginine kinase and creatine kinase afforded non-specific enzyme patterns in the species tested. Adenylate kinase and malate dehydrogenase did not give specific or reproducible enzyme patterns. Analysis of LDH and G-3-PD activities in commercial IEF gels was shown to be a reliable and reproducible technique for distinguishing closely related Merluccius spp. and other gadoid fish specieses_ES
dc.description.sponsorshipThe authors thank the financial support of CICYT (Project ALI95-0053)es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsopenAccesses_ES
dc.subjectGadoid fish species identificationes_ES
dc.subjectEnzyme detectiones_ES
dc.subjectIsoelectric focusinges_ES
dc.titleSpecific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish specieses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/S0308-8146(00)00042-X-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/S0308-8146(00)00042-Xes_ES
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