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Title

Species identification of smoked and gravad fish products by sodium dodecylsulphate polyacrylamide gel electrophoresis, urea isoelectric focusing and native isoelectric focusing: A collaborative study

AuthorsMackie, I. M.; Pérez Martín, Ricardo Isaac ; Piñeiro, Carmen
Issue Date2000
PublisherElsevier
CitationFood Chemistry 71(1): 1-7 (2000)
AbstractA collaborative study on the use of sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), urea-isoelectric focusing (urea-IEF) and native isoelectric focusing for the identi®cation of species of smoked salmonids, gravad salmonids and smoked eels was carried out by eight laboratories. With SDS-PAGE, minor changes took place in the pro®les of the processed salmonid species making it impossible or very di cult to identify closely related species. With urea-IEF, there were fewer changes in the pro®les due to processing and the system generally had greater species-discriminating power for the processed salmonids than SDS-PAGE. The pro®les of the eel species as obtained on SDS-PAGE or urea-IEF were not a ected by smoking. Urea-IEF had greater species-discriminating power than SDS-PAGE for the eel species. Native IEF was useful in providing supplementary iden- ti®cation on species di cult to identify by SDS-PAGE or by urea-IEF in the case of cold smoked products.
Description7 páginas, 6 figuras, 2 tablas.-- I. Mackie ... et al.
Publisher version (URL)http://dx.doi.org/10.1016/S0308-8146(00)00147-3
URIhttp://hdl.handle.net/10261/57035
DOI10.1016/S0308-8146(00)00147-3
ISSN0308-8146
Appears in Collections:(IIM) Artículos
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