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Fish muscle parvalbumins as marker proteins for native and urea isoelectric focusing

AuthorsRehbein, Hartmut; Kündiger, R.; Piñeiro, Carmen ; Pérez Martín, Ricardo Isaac
Isoelectric focusing
Marker proteins
Issue Date2000
PublisherJohn Wiley & Sons
CitationElectrophoresis 21(8): 1458-1463 (2000)
AbstractAn isoelectric point (pI) calibration kit containing fish muscle parvalbumins was prepared and tested for its suitability for isoelectric focusing (IEF) in the presence of 8 M urea. The pattern obtained by urea CleanGel IEF consisted of nine bands covering the pI range 4.96—5.64. This range is relevant for species identification of heated fish by urea IEF. The kit may also be used for native IEF in the low pH range, as demonstrated by running an extract made from the kit together with water-soluble fish muscle proteins on Servalyt Precotes 3—6.
Description6 páginas, 4 tablas, 6 figuras
Publisher version (URL)http://dx.doi.org/10.1002/(SICI)1522-2683(20000501)21:8<1458::AID-ELPS1458>3.0.CO;2-T
Appears in Collections:(IIM) Artículos
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