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Silencing supresor activity of the Tobacco Rattle Virus-encoded 16-kDa protein and interference with endogenous small RNA-guided regulatory pathways

AuthorsMartínez-Priego, Llucia ; Donaire, Livia ; Barajas, Daniel; Llave, César
KeywordsRNA silencing
Plant virus
Viral suppressor
Small RNA
Issue Date5-Jul-2008
CitationVirology 376(2): 346-356(2008)
AbstractHigher plants use RNA silencing as a defense mechanism against viral infections, but viruses may encode suppressor proteins that counteract these defenses. Several virus-encoded suppressors also exert an inhibitory effect on endogenous small RNA regulatory pathways. Here we characterized the Tobacco rattle virus-encoded 16-kDa (TRV-16K) protein as a suppressor that blocked local RNA silencing induced by single (s)- and double-stranded (ds) RNA, indicating that TRV-16K interfered with a step in the silencing pathway downstream of dsRNA formation. The suppressor activity of TRV-16K was severely compromised by moderate to high dosages of dsRNA inducer. When silencing was locally triggered by ssRNA or low levels of dsRNA, silencing suppression by TRV-16K was associated with reduced accumulation of silencing-related siRNAs. TRV-16K also prevented partially cell-to-cell movement and systemic propagation of silencing but not transitive amplification of RNA silencing. We showed that neither TRV nor TRV-16K caused a global deregulation of the microRNA-regulatory pathway in Arabidopsis, suggesting that interference with microRNA biology was not a prerequisite for TRV, and probably many other plant viruses, to develop systemic infections in plants
Description11 páginas, 6 figuras -- PAGS nros. 346-356
Publisher version (URL)http://dx.doi.org/10.1016/j.virol.2008.03.024
Appears in Collections:(CIB) Artículos
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