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dc.contributor.authorMartínez-Priego, Llucia-
dc.contributor.authorDonaire, Livia-
dc.contributor.authorBarajas, Daniel-
dc.contributor.authorLlave, César-
dc.identifier.citationVirology 376(2): 346-356(2008)es_ES
dc.description11 páginas, 6 figuras -- PAGS nros. 346-356es_ES
dc.description.abstractHigher plants use RNA silencing as a defense mechanism against viral infections, but viruses may encode suppressor proteins that counteract these defenses. Several virus-encoded suppressors also exert an inhibitory effect on endogenous small RNA regulatory pathways. Here we characterized the Tobacco rattle virus-encoded 16-kDa (TRV-16K) protein as a suppressor that blocked local RNA silencing induced by single (s)- and double-stranded (ds) RNA, indicating that TRV-16K interfered with a step in the silencing pathway downstream of dsRNA formation. The suppressor activity of TRV-16K was severely compromised by moderate to high dosages of dsRNA inducer. When silencing was locally triggered by ssRNA or low levels of dsRNA, silencing suppression by TRV-16K was associated with reduced accumulation of silencing-related siRNAs. TRV-16K also prevented partially cell-to-cell movement and systemic propagation of silencing but not transitive amplification of RNA silencing. We showed that neither TRV nor TRV-16K caused a global deregulation of the microRNA-regulatory pathway in Arabidopsis, suggesting that interference with microRNA biology was not a prerequisite for TRV, and probably many other plant viruses, to develop systemic infections in plantses_ES
dc.description.sponsorshipThis work was supported by Grants GEN2003-20222-C02-01 and BIO2006-13107 from the MEC (Spain) and GR/SAL/0821/2004 from the CAM (Spain)es_ES
dc.subjectRNA silencinges_ES
dc.subjectPlant viruses_ES
dc.subjectViral suppressores_ES
dc.subjectSmall RNAes_ES
dc.titleSilencing supresor activity of the Tobacco Rattle Virus-encoded 16-kDa protein and interference with endogenous small RNA-guided regulatory pathwayses_ES
dc.description.peerreviewedPeer reviewedes_ES
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