Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/56500
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Competition saturation transfer difference experiments improved with isotope editing and filtering schemes in NMR-based screening |
Autor: | Fehér, K.; Groves, Patrick CSIC ORCID; Batta, Gyula; Jiménez-Barbero, Jesús CSIC ORCID; Muhle-Goll, Claudia; Kövér, Katalin E. | Fecha de publicación: | 17-dic-2008 | Editor: | American Chemical Society | Citación: | Journal of the American Chemical Society 130 (50) : 17148-17153 (2008) | Resumen: | Competition binding experiments are used in NMR-based screenings to match up to the binding site with that of a known ligand and to determine the strength of the interaction, thus providing a ranking of hits according to receptor affinity. These competition titration experiments must use a reference ligand for which the binding site on the receptor and the affinity of the interaction is known. These experiments rely on the observation of separate signals of the reference and hit compounds, which is very often hampered by signal overlap. Here, we present a combination of isotope editing and filtering schemes with saturation transfer difference (STD) experiments that allows the separation of the STD signals of the labeled reference ligand from that of the natural abundance hit compound even in the case of severe signal overlap. Thus, the measurement of well-defined titration curves in competition STD titration experiments is feasible and allows the quantitative determination of binding constants. Note that the method requires the availability of the reference ligand in an NMR-active, stable-isotope-labeled form | Descripción: | 6 páginas, 5 figuras, 1 esquema -- PAGS nros. 17148-17153 | Versión del editor: | http://dx.doi.org/10.1021/ja804468k | URI: | http://hdl.handle.net/10261/56500 http://hdl.handle.net/10261/59500 |
DOI: | 10.1021/ja804468k | ISSN: | 0002-7863 | E-ISSN: | 1520-5126 |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
restringido.pdf | 21,67 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
23
checked on 10-abr-2024
WEB OF SCIENCETM
Citations
21
checked on 18-feb-2024
Page view(s)
354
checked on 18-abr-2024
Download(s)
265
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.