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Title

Differentiation of raw or processed eel by PCR-based techniques: Restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP)

AuthorsRehbein, Hartmut; González Sotelo, Carmen ; Pérez Martín, Ricardo Isaac
KeywordsEel
Species identification
PCR-RFLP
PCR-SSCP
Issue Date2002
PublisherSpringer
CitationEuropean Food Research and Technology 214(2): 171-177 (2002)
AbstractPCR-based methods have been developed to differentiate four species of commercially important eels (Anguilla anguilla, A. rostrata, A. japonica, A. australis). By means of universal primers a 464 base pair (bp) section of the cytochrome b gene was amplified. The amplicon was sequenced and restriction enzymes were selected for RFLP analysis; a short part (123 bp) of the 464 bp section was amplified by another set of primers and used for SSCP analysis. The suitability of RFLP and SSCP for differentiating hot-smoked eel was verified by a collaborative study.
Description7 páginas, 7 figuras, 3 tablas.-- Hartmut Rehbein ... et al.
Publisher version (URL)http://link.springer.com/static-content/0.5284/pdf/871/art%253A10.1007%252Fs00217-001-0457-y.pdf?token=1348052952593--539e628645773687179ff3549e8c820735c781b208b4167e4dd19b7d6c62b9741d6e33ed36d9eb50d2baf326c32ea34190d82f7d7273e33768c2978e570927c9&doi=10.1007/s00217-001-0457-y&contentType=article
URIhttp://hdl.handle.net/10261/56478
ISSN1438-2377
E-ISSN1438-2385
Appears in Collections:(IIM) Artículos
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