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Expression profile of pha gene cluster of Pseudomonas putida KT2442

AuthorsEugenio, Laura I. de; Escapa, Isabel F.; Galán, Beatriz ; García, José Luis ; Prieto, María Auxiliadora
KeywordsPHA production
transcriptional regulation
Issue DateAug-2008
CitationMacromolecular Symposia, 269 (1) : 8-10 (2008)
AbstractPolyhydroxyalkanoates (PHAs) are biodegradable polymers that many bacteria accumulate as carbon and energy storage when growth conditions are unbalanced. Pseudomonas strains belonging to the rRNA homology group I such as P. putida can accumulate medium-chain-length-PHA from monomers in the C8 to C10 range. Regulation of PHA synthesis and degradation in P. putida KT2442 has been studied using different molecular approaches. In this study six promoter regions located upstream of each pha gene were identified. The expression of the pha cluster have been analysed in the presence of octanoic acid versus glucose in the culture medium. Results demonstrated that the system is activated in the presence of octanoic acid as PHA precursor
Description3 páginas, 3 figuras -- PAGS nros. 8-10
Publisher version (URL)http://dx.doi.org/10.1002/masy.200850902
Appears in Collections:(CIB) Artículos
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