Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/56132
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Age-related changes in membrane lipid composition, fluidity and respiratory burst in rat peritoneal neutrophils

AutorÁlvarez, E.; Ruiz-Gutiérrez, Valentina CSIC ; Sobrino Castelló, Francisco CSIC ORCID; Santa-María, Consuelo
Palabras claveNeutrophil
Respiratory burst
Membrane fluidity
Lipid composition
Fecha de publicaciónabr-2001
EditorJohn Wiley & Sons
CitaciónClinical and Experimental Immunology 124(1): 95-102 (2001)
ResumenThe O·2−. production has been studied in rat peritoneal neutrophils of different age (3, 12 and 24 months), in order to analyse whether the neutrophil respiratory burst is modified with increasing age. To stimulate NADPH oxidase, the enzyme responsible for the respiratory burst, two stimuli that act in different way have been used: phorbol myristate acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (N-FMLP). Production of O·2− decreased with age in neutrophils stimulated with N-FMLP (about 40%), but not in the stimulated with PMA. No difference in NADPH oxidase activity was found with age. The NADPH is supplied to the respiratory burst mainly by the pentose phosphate shunt. A progressive and significant decrease in the two most important enzymes of this route, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, was detected as a function of age; in spite of this reduction, the NADPH produced by cells from old animals seems not limiting for the O·2− production. The N-FMLP-induced decrease in the O·2− production may be related to the age-dependent increase in the membrane fluidity observed. A decline in the cholesterol/phospholipid ratio and a rise in the total polyunsaturated fatty acids content were found, that correlated well with the increase in the membrane fluidity. The decrease (50%) of phosphatidylinositols in the 24-month-old animals may be also related to the age-impairment in the respiratory burst found after stimulation with N-FMLP. These studies suggest that the age-related alterations in neutrophil may result in diminished neutrophil function and increased susceptibility to infection in the ageing.
Versión del editorhttp://dx.doi.org/10.1046/j.1365-2249.2001.01490.x
URIhttp://hdl.handle.net/10261/56132
DOI10.1046/j.1365-2249.2001.01490.x
ISSN0009-9104
E-ISSN1365-2249
Aparece en las colecciones: (IG) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

12
checked on 27-mar-2024

SCOPUSTM   
Citations

56
checked on 21-mar-2024

WEB OF SCIENCETM
Citations

41
checked on 26-feb-2024

Page view(s)

342
checked on 27-mar-2024

Download(s)

106
checked on 27-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.