English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/56122
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Thyroid hormone regulation of gene expression in the developing rat fetal cerebral cortex: Prominent role of the Ca2+/calmodulin-dependent protein kinase IV pathway

AuthorsMorte, Beatriz ; Díez, Diego ; Ausó Monreal, Eva; Belinchón, Mónica M.; Gil-Ibáñez, Pilar ; Grijota Martínez, María del Carmen; Navarro, Daniela; Morreale de Escobar, Gabriella ; Berbel, Pere; Bernal, Juan
Issue DateFeb-2010
PublisherEndocrine Society
CitationEndocrinology 151(2): 810-820 (2010)
AbstractThyroid hormones influence brain development through regulation of gene expression mediated by nuclear receptors. Nuclear receptor concentration increases rapidly in the human fetus during the secondtrimester,aperiod of high sensitivity of the brain to thyroidhormones.In the rat, the equivalent period is the last quarter of pregnancy. However, little is known about thyroid hormone action in the fetal brain, and in rodents, most thyroid hormone-regulated genes have been identified during the postnatal period. To identify potential targets of thyroid hormone in the fetal brain,weinduced maternal and fetal hypothyroidism by maternal thyroidectomy followed by antithyroid drug (2-mercapto-1-methylimidazole) treatment. Microarray analysis identified differentially expressed genes in the cerebral cortex of hypothyroid fetuses on d 21 after conception. Gene function analysis revealed genes involved in the biogenesis of the cytoskeleton, neuronal migration and growth,and branching of neurites. Twenty percent of the differentially expressed genes were related to each other centered on the Ca2 and calmodulin-activated kinase (Camk4) pathway.Camk4was regulated directly by T3 in primary cultured neurons from fetal cortex, and the Camk4 protein was also induced by thyroid hormone. No differentially expressed genes were recovered when euthyroid fetuses from hypothyroid mothers were compared with fetuses from normal mothers. Although the resultsdonot rule out a specific contribution from the mother, especially at earlier stages of pregnancy, they indicate that the main regulators of thyroid hormone-dependent, fetal brain gene expression near term are the fetal thyroid hormones.
Description11 p., 6 figures, 1 table and references.
Publisher version (URL)http://dx.doi.org/10.1210/en.2009-0958
URIhttp://hdl.handle.net/10261/56122
DOI10.1210/en.2009-0958
ISSN0013-7227
E-ISSN1945-7170
Appears in Collections:(IN) Artículos
(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
Thyroid hormone regulation of gene.pdf254,76 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.