English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/56102
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Ric-3 promotes α7 nicotinic receptor assembly and trafficking through the ER subcompartment of dendrites

AuthorsAlexander, John K,; Sagher, Daphna; Krivoshein, Arcadius V.; Criado, Manuel; Jefford, Gregory; Green, William N.
Issue DateJul-2010
PublisherSociety for Neuroscience
CitationJournal of Neuroscience 30(30): 10112-10126 (2010)
AbstractThe function of Ric-3, which is required for nicotinic acetylcholine receptor (nAChR) expression in C. elegans, is unclear. Here we found that Ric-3 can promote or inhibit cell-surface delivery of α-bungarotoxin-binding nAChRs (BgtRs) composed of α7 subunits. At low levels, Ric-3 promoted BgtR assembly, endoplasmic reticulum (ER) release, and cell-surface delivery without trafficking from the ER. At high Ric-3 levels, Ric-3 suppressed BgtR surface delivery, but not its assembly, and BgtRs were retained in the ER or in Ric-3-containing aggregates. In PC12 cells, native BgtRs trafficked to the cell surface from the ER where low levels of endogenous Ric-3 were observed. In cultured neurons, native Ric-3 levels were higher than in PC12 cells, and Ric-3 and α7 subunits were found in somata and dendrites, but not axons, of inhibitory interneurons. Ric-3 trafficked with α7 subunits in rapidly moving vesicles to dendrites, where it was restricted to the ER subcompartment. We conclude that Ric-3 has two potential functions. At low levels, Ric-3 interactions are short-lived and promote BgtR assembly and ER release. At higher levels, Ric-3 interactions are longer-lived and mediate ER retention. In neurons, Ric-3 ER retention appears to promote transport within the dendritic ER subcompartment, thereby restricting α7 trafficking to dendrites and preventing axonal transport.
Description33 p., 9 figures, 1 table and references.
Publisher version (URL)http://dx.doi.org/10.1523/JNEUROSCI.6344-09.2010
URIhttp://hdl.handle.net/10261/56102
DOI10.1523/JNEUROSCI.6344-09.2010
E-ISSN1529-2401
Appears in Collections:(IN) Artículos
Files in This Item:
File Description SizeFormat 
Ric-3 Promotes alpha 7.pdf6,74 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.