English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/56018
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Actomyosin contraction at the cell rear drives nuclear translocation in migrating cortical interneurons

AuthorsMartini, Francisco J.; Valdeolmillos, Miguel
Issue DateJun-2010
PublisherSociety for Neuroscience
CitationJournal of Neuroscience 30(25): 8660-8670 (2010)
AbstractNeuronal migration is a complex process requiring the coordinated interaction of cytoskeletal components and regulated by calcium signaling among other factors. Migratory neurons are polarized cells in which the largest intracellular organelle, the nucleus, has to move repeatedly. Current views support a central role for pulling forces that drive nuclear movement. The participation of actomyosin driven forces acting at the nucleus rear has been suggested, however its precise contribution has not been directly addressed. By analyzing interneurons migrating in cortical slices of mouse brains, we have found that nucleokinesis is associated with a precise pattern of actin dynamics characterized by the initial formation of a cup-like actin structure at the rear nuclear pole. Time-lapse experiments show that progressive actomyosin contraction drives the nucleus forward. Nucleokinesis concludes with the complete contraction of the cup-like structure, resulting in an actin spot at the base of the retracting trailing process. Our results demonstrate that this actin remodeling requires a threshold calcium level provided by low-frequency spontaneous fast intracellular calcium transients. Microtubule stabilization with taxol treatment prevents actin remodeling and nucleokinesis, whereas cells with a collapsed microtubule cytoskeleton induced by nocodazole treatment, display nearly normal actin dynamics and nucleokinesis. In summary, the results presented here demonstrate that actomyosin forces acting at the rear side of the nucleus drives nucleokinesis in tangentially migrating interneurons in a process that requires calcium and a dynamic cytoskeleton of microtubules.
Description11 p., 7 figures and references.
Publisher version (URL)http://dx.doi.org/10.1523/JNEUROSCI.1962-10.2010
URIhttp://hdl.handle.net/10261/56018
DOI10.1523/JNEUROSCI.1962-10.2010
ISSN0270-6474
Appears in Collections:(IN) Artículos
Files in This Item:
File Description SizeFormat 
Actomyosin Contraction at the Cell Rear Drives Nuclear.pdf3,62 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.