English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/55466
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Lectin-binding pattern of the senegalese sole Solea senegalensis oogenesis

AuthorsAccogli, Gianluca; Zizza, Sara; García-López, Ángel ; Sarasquete, Carmen ; Desantis, Salvatore
Lectin histochemistry
Solea senegalensis
Issue DateAug-2012
PublisherJohn Wiley & Sons
CitationMicroscopy Research and Technique 75(8): 1124-1135 (2012)
AbstractThe glycoconjugate pattern of developing ovarian follicles in wild and cultured Senegalese sole Solea senegalensis was investigated by means of lectin histochemistry. Ovaries from cultured fish contained oocytes up to the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. The follicular cells bound MAL II, SBA, HPA, DBA, Con A, KOH-sialidase (K-s)-WGA, GSA I-B4 in the late vitellogenic stage, and in wild fish also SNA and K-s-PNA, whereas in the hydration stage SBA, HPA, DBA, and GSA I-B4 only. The zona radiata reacted with SBA, HPA, DBA, Con A, and GSA I B4 in the late vitellogenic stage and in cultured fish also with UEA I, whereas in the hydration stage it stained with SBA only. The cortical alveoli bound SBA, HPA, RCA120 during the late vitellogenic stage, also SNA, PNA, K-s-PNA, GSA I-B4 in cultured fish, DBA, and K-s-WGA in wild ones which stained with SBA, HPA, and GSA I-B4 in the hydrated stage. The yolk reacted with Con A in the late vitellogenic oocytes, and also with MAL II, SNA, K-s-PNA, SBA, HPA, K-s-WGA, GSA I-B4, UEA I in the hydrated ones. From perinucleolus to late vitellogenic stages, the oocyte nucleoplasm bound Con A, GSA I-B4, GSA II, UEA I, and in wild fish also MAL II, SNA, LTA but only GSA I-B4 reactivity in the early maturation stage. These findings demonstrate that the glycan pattern of fish ovarian follicles changes during the maturative stages and that it is affected by culture-rearing conditions.
Publisher version (URL)http://dx.doi.org/10.1002/jemt.22040
Appears in Collections:(ICMAN) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.