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Title

Trends in sample preparation for classical and second generation proteomics

AuthorsCañas, Benito; Piñeiro, Carmen ; Calvo, Enrique; López-Ferrer, Daniel; Gallardo, José Manuel
KeywordsProteomics
Sample preparation
2-DE
ESI
MALDI
Protein digestion
Issue Date2007
PublisherElsevier
CitationJournal of Chromatography A 1153(1-2): 235-258 (2007)
AbstractSample preparation is a fundamental step in the proteomics workflow. However, it is not easy to find compiled information updating this subject. In this paper, the strategies and protocols for protein extraction and identification, following either classical or second generation proteomics methodologies, are reviewed. Procedures for: tissue disruption, cell lysis, sample pre-fractionation, protein separation by 2-DE, protein digestion, mass spectrometry analysis, multidimensional peptide separations and quantification of protein expression level are described.
Description24 páginas, 2 figuras, 3 tablas
Publisher version (URL)http://dx.doi.org/10.1016/j.chroma.2007.01.045
URIhttp://hdl.handle.net/10261/55460
DOI10.1016/j.chroma.2007.01.045
ISSN0021-9673
Appears in Collections:(IIM) Artículos
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