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dc.contributor.authorPascoal, Ananías-
dc.contributor.authorBarros-Velázquez, J.-
dc.contributor.authorCepeda, Alberto-
dc.contributor.authorGallardo, José Manuel-
dc.contributor.authorCalo-Mata, Pilar-
dc.identifier.citationElectrophoresis 29(2): 499-509 (2008)es_ES
dc.description11 páginas, 6 figuras, 2 tablases_ES
dc.description.abstractA simple PCR-RFLP method has been developed for the identification of 19 penaeid shrimp species of food interest belonging to the superfamily Penaeoidea. Preliminary amplification, sequencing and alignment of a 960 bp fragment of the 16S rRNA/tRNAVal/12S rRNA mitochondrial region allowed the design of 16Scru4/16Scru4 primers, constructed on well-conserved mitochondrial sequences of the penaeid shrimp species considered. Such primers afforded the amplification of an internal 515–535 bp region of the 16S rRNA/tRNAVal genes that, when subjected to cleavage with AluI, TaqI and HinfI, provided species-specific restriction patterns. Moreover, the proposed method also allowed the definition of different intraspecific restriction types between different populations of Litopenaeus vannamei, Farfantepenaeus notialis, Fenneropenaeus merguiensis, Metapenaeus sp., Melicertus latisulcatus and Pleoticus muelleri of different origins. The method described here was also successfully applied for the identification of penaeid shrimps in complex processed precooked foods, where this type of shellfish is used as an added-value food ingredient. Sequencing analysis provided new information about the genetic relationships among shrimps not only at the levels of species and genus, but also among different populations at intraspecific level. The 16S rRNA/tRNAVal fragment considered in this study seems to be accurate for shrimp species identification in raw and processed foodstuffs and for phylogenetic analysis among penaeid shrimp specieses_ES
dc.description.sponsorshipThe authors thank the financial support from the National Food Program of the INIA (Spanish Ministry for Education) (Project CAL-03-030- C2-1) and from the PGIDIT Research Program in Marine Resources (Project PGIDIT04RMA261004PR) of the Xunta de Galicia (Galician Council for Industry Commerce and Innovation).es_ES
dc.subjectFood authenticityes_ES
dc.subjectPenaeid shrimpses_ES
dc.subjectSpecies identificationes_ES
dc.titleA polymerase chain reaction-restriction fragment length polymorphism method based on the analysis of a 16S rRNA/tRNAVal mitochondrial region for species identification of commercial penaeid shrimps (Crustacea: Decapoda: Penaeoidea) of food interestes_ES
dc.description.peerreviewedPeer reviewedes_ES
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