English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/55064
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Real-time PCR detection and quantification of fish probiotic Phaeobacter strain 27-4 and fish pathogenic Vibrio in microalgae, rotifer, Artemia and first feeding turbot (Psetta maxima) larvae

AuthorsProl García, María Jesús ; Bruhn, J. B.; Pintado, José ; Gram, Lone
KeywordsMicroalgae
Phaebobacter
Real-time PCR
Turbot larval probiotics
Vibrio
Issue Date2009
PublisherWiley-Blackwell
CitationJournal of Applied Microbiology 106(4): 1292-1303 (2009)
AbstractAims: To develop a SYBR Green quantitative real-time PCR protocol enabling detection and quantification of a fish probiotic and two turbot pathogenic Vibrio spp. in microcosms. Methods and Results: Phaeobacter 27-4, Vibrio anguillarum 90-11-287 and Vibrio splendidus DMC-1 were quantified as pure and mixed cultures and in presence of microalgae (Isochrysis galbana), rotifers (Brachionus plicatilis), Artemia nauplii or turbot (Psetta maxima) larvae by real-time PCR based on primers directed at genetic loci coding for antagonistic and virulence-related functions respectively. The optimized protocol was used to study bioencapsulation and maintenance of the probiont and pathogens in rotifers and for the detection and quantification of Phaeobacter and V. anguillarum in turbot larvae fed rotifers loaded with the different bacteria in a challenge trial. Conclusions: Our real-time PCR protocol is reproducible and specific. The method requires separate standard curve for each host organism and can be used to detect and quantify probiotic Phaeobacter and pathogenic Vibrio bioencapsulated in rotifers and in turbot larvae. Significance and Impact of the Study: Our method allows monitoring and quantification of a turbot larvae probiotic bacteria and turbot pathogenic vibrios in in vivo trials and will be useful tools for detecting the bacteria in industrial rearing units
Description12 páginas, 5 tablas, 1 figura
Publisher version (URL)http://dx.doi.org/10.1111/j.1365-2672.2008.04096.x
URIhttp://hdl.handle.net/10261/55064
DOI10.1111/j.1365-2672.2008.04096.x
ISSN1364-5072
E-ISSN1365-2672
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.