Development and cytogenetic characterisation of non-brittle rachis tritordeum lines

Abstract

xTritordeum (Ascherson et Graebner, an amphiploid between Triticum turgidum conv. durum and Hordeum chilense), and a set of chromosome substitution lines of tritordeum, where 2Hch or 3Hch H. chilense chromosomes were replaced with 2D chromosome of T. aestivum or 3Hv chromosome of H. vulgare, were used to assess the effect of the specific chromosome on brittle rachis in tritordeum. Tritordeum has brittle rachis. In contrast, the 2D(2Hch) and 3Hv(3Hch) substitution lines showed non-brittle rachis, and they also have tenacious glumes and compact spikes which are excellent characters to improve the tritordeum threshing. We have also developed different combinations between 2D and 3Hv translocation lines in tritordeum. We present in this paper the identification and characterisation of all these introgression lines in tritordeum by fluorescent in situ hybridisation.