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Title

Expression of Mytilus immune genes in response to experimental challenges varied according to the site of collection

AuthorsLi, Hui; Venier, Paola; Prado-Álvarez, María ; Gestal, C. ; Toubiana, Mylène; Quartesan, Rosita; Borghesan, Fabio; Novoa, Beatriz ; Figueras Huerta, Antonio ; Figueras Huerta, Antonio ; Roch, Philippe
KeywordsAntimicrobial peptide
Lysozyme
HSP70
Gene regulation
Quantitative PCR
ISH
Vibrio
Heat shock
Innate immunity
Mytilus
Molluscs
Issue Date2010
PublisherElsevier
CitationFish and Shellfish Immunology 28(4): 640-648 (2010)
AbstractMussels live in diverse coastal environments experience various physical, chemical and biological conditions, which they counteract with functional adjustments and heritable adaptive changes. In order to investigate possible differences in immune system capabilities, we analyzed by qPCR the expression levels of 4 immune genes (defensin, mytilin B, myticin B, lysozyme) and HSP70 in the Mediterranean mussel, Mytilus galloprovincialis collected in 3 European farming areas {Atlantic Ocean-Ría de Vigo-Spain (RV), French Mediterranean Gulf of Lion-Palavas-Prévost lagoon (PP) and Northern Adriatic Sea-Venice-Italy (VI)} in response to one injection of one of the 3 bacterial species (Vibrio splendidus LGP32, Vibrio anguillarum, Micrococcus lysodeikticus), and to heat shock or cold stress. We confirmed that the 5 genes are constitutively expressed in hemocytes, defensin being the less expressed, myticin B the highest. As suspected, the same gene resulted differently expressed according to mussel group, with the biggest difference being for HSP70 and lysozyme and lowest expression of all the 5 genes in mussels from RV. In addition, gene expression levels varied according to the challenge. Most frequent effect of bacterial injections was down-regulation, especially for mytilin B and myticin B. Heat shock enhanced transcript levels, particularly in mussels from RV, whereas cold stress had no effect. In situ hybridization of labelled probes on mussel hemocytes indicated that bacterial injections did not change the mRNA patterns of defensin and myticin B whereas mytilin B mRNA almost disappeared. In conclusion, these results demonstrated that constitutive level, nature and intensity of immune gene expression regulations strongly depended from mussel group, and support the concept of gene–environment interactions
Description9 páginas, 5 figuras
Publisher version (URL)http://dx.doi.org/10.1016/j.fsi.2009.12.022
URIhttp://hdl.handle.net/10261/54891
DOI10.1016/j.fsi.2009.12.022
ISSN1050-4648
Appears in Collections:(IIM) Artículos
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