English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/54833
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Pediococcus acidilactici in the culture of turbot (Psetta maxima) larvae: Administration pathways

AuthorsVillamil, L.; Figueras Huerta, Antonio ; Planas, Miguel ; Novoa, Beatriz
KeywordsProbiotic
Pediococcus acidilactici
Turbot larvae
Psetta maxima
Brachionus plicatilis
Bio-encapsulation
Issue Date2010
PublisherElsevier
CitationAquaculture 307(1-2): 83-88 (2010)
AbstractProbiotic administration to marine fish larvae and live prey has been shown to be a useful means to prevent bacterial infection and mass mortalities. Here, several experiments were carried out in order to determine the optimum protocol for Pediococcus acidilactici delivery to the turbot (Psetta maxima) gastrointestinal tract by its administration via rotifers supplemented for 1 or 24 h with the bacteria or directly via the water. The survival capacity of P. acidilactici in sterile seawater, in microalgae (Isochrysis galbana) culture and in rotifers (Brachionus plicatilis) was determined, with best results in rotifer culture. The incorporation of P. acidilactici into the rotifer's gut was time-dependant, with P. acidilactici recovery from rotifer cultures supplemented with the lactic acid bacteria suspension for 24 h being significantly lower than from cultures supplemented for 1 h. Similarly, P. acidilactici recovery from the gastrointestinal tract of turbot larvae was higher when larvae were fed with 1-h supplemented rotifer cultures. However, significantly higher P. acidilactaci recovery from turbot larvae gastrointestinal tract was observed when the probiotic was directly administered in the rearing water. Lastly, the bactericidal activity of the extratracellular products (ECPs) of P. acidilactici against a pathogenic bacterium for turbot, Vibrio splendidus (Hm 112) was assessed. The ECPs of P. acidilactici significantly inhibited the growth of the pathogenic bacteria in vitro. However, during in vivo experiments, the bacterial community, dominated mainly by Vibrio and Pseudomonas, was not apparently affected by P. acidilactici supplementation in rotifers or turbot larvae.
Description6 páginas, 4 figuras
Publisher version (URL)http://dx.doi.org/10.1016/j.aquaculture.2010.07.004
URIhttp://hdl.handle.net/10261/54833
DOI10.1016/j.aquaculture.2010.07.004
ISSN0044-8486
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.