English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/54461
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Molecular identification of the black tiger shrimp (Penaeus monodon), the white leg shrimp (Litopenaeus vannamei) and the Indian white shrimp (Fenneropenaeus indicus) by PCR targeted to the 16S rRNA mtDNA

AuthorsPascoal, Ananías; Barros-Velázquez, J. ; Ortea, Ignacio ; Cepeda, Alberto; Gallardo, José Manuel ; Calo-Mata, Pilar
KeywordsSpecies identification
Penaeid shrimps
Prawns
16S rRNA
mtDNA
PCR
Food authenticity
Food labelling
Decapoda crustaceans
Issue Date2011
PublisherElsevier
CitationFood Chemistry 125(4): 1457-1461 (2011)
AbstractPolymerase chain reaction-based methodologies have been developed for the identification of three commercially- relevant penaeid shrimp species, these were: Litopenaeus vannamei, Penaeus monodon and Fenneropenaeus indicus in food products. Such three species represent more than 80% of the whole farmed shrimp production worldwide and may be fraudulently replaced by species exhibiting lower value such as Litopenaeus stylirostris, Penaeus semisulcatus and Fenneropenaeus merguiensis, respectively. For it, preliminary sequencing of a mitochondrial sequence of ca. 530 bp in the 16S rRNA/tRNAVal mitochondrial region was performed in nearly 20 penaeid shrimp species of commercial relevance. Careful analysis of such sequences allowed the design of primers PNVF/PNVR, which allowed the combined identification of P. monodon and L. vannamei, and PNIF/PNIR, which allowed the specific identification of F. indicus. In addition, P. monodon and L. vannamei could be easily differentiated by either restriction with TspE1 or by amplification with novel primers MPNF/MPNR, specific for P. monodon. The proposed specific methods improve current general identification methods of these species based on more general RFLP analyses. In addition, these methods can be easily completed in less than 8 h.
Description5 páginas, 2 figuras, 2 tablas
Publisher version (URL)http://dx.doi.org/10.1016/j.foodchem.2010.10.053
URIhttp://hdl.handle.net/10261/54461
DOI10.1016/j.foodchem.2010.10.053
ISSN0308-8146
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.