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dc.contributor.authorGalán, Beatriz-
dc.contributor.authorManso, Isabel-
dc.contributor.authorKolb, Annie-
dc.contributor.authorGarcía, José Luis-
dc.contributor.authorPrieto, María Auxiliadora-
dc.identifier.citationMicrobiology 154(7):2151-2160(2008)es_ES
dc.description10 páginas, 6 figuras, 1 tabla -- PAGS nros. 2151-2160es_ES
dc.description.abstractExpression from the Escherichia coli W meta-hpa operon promoter (Pg) is under a strict catabolic repression control mediated by the cAMP-catabolite repression protein (CRP) complex in a glucose-containing medium. The Pg promoter is also activated by the integration host factor (IHF) and repressed by the specific transcriptional regulator HpaR when 4-hydroxyphenylacetate (4HPA) is not present in the medium. Expression from the hpa promoter is also repressed in undefined rich medium such as LB, but the molecular basis of this mechanism is not understood. We present in vitro and in vivo studies to demonstrate the involvement of FIS protein in this catabolic repression. DNase I footprinting experiments show that FIS binds to multiple sites within the Pg promoter. FIS-site I overlaps the CRP-binding site. By using an electromobility shift assay, we demonstrated that FIS efficiently competes with CRP for binding to the Pg promoter, suggesting an antagonist/competitive mechanism. RT-PCR showed that the Pg repression effect is relieved in a FIS deleted strain. The repression role of FIS at Pg was further demonstrated by in vitro transcription assays. These results suggest that FIS contributes to silencing the Pg promoter in the exponential phase of growth in an undefined rich medium when FIS is predominantly expressedes_ES
dc.description.sponsorshipThis work was supported by Comunidad Autónoma de Madrid Grant AMB-259-0505, by Comisión Interministerial de Ciencia y Tecnología Grants BIO2007-67304-C02 and CTM2006-04007, by European Union Grants GEN 2006-27750-C5-3-E and NMP2-CT-2007-026515, and by the Programme de Recherche Fondamentale en Microbiologie, Maladies Infectieuses et Parasitaireses_ES
dc.publisherSociety for General Microbiologyes_ES
dc.subjectCRP, catabolite repression proteines_ES
dc.subjectEMSA, electromobility shift assayes_ES
dc.subjectFIS, factor for inversion stimulationes_ES
dc.subjectHPA, hydroxyphenylacetatees_ES
dc.subjectHPC, 3,4-dihydroxyphenylacetic acides_ES
dc.subjectIHF, integration host factores_ES
dc.subjectRNAP, RNA polymerasees_ES
dc.subjectRT-PCR, reverse transcriptase PCRes_ES
dc.titleThe role of FIS protein in the physiological control of the expression of the Escherichia coli meta-hpa operones_ES
dc.description.peerreviewedPeer reviewedes_ES
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