English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/53623
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Plasmid replication initiator RepB forms a hexamer reminiscent of ring helicases and has mobile nuclease domains

AuthorsBoer, Roeland ; Ruiz-Masó, José A. ; López-Blanco, José R.; Chacón, Pablo ; Usón, Isabel ; Espinosa, Manuel ; Llorca, Óscar ; Solar, Gloria del
KeywordsDNA-binding protein
Nuclease
Plasmid replication
Replication initiator
X-ray crystal structure
Issue Date3-Jun-2009
CitationEMBO Journal, 28: 1666-1678 (2009)
AbstractRepB initiates plasmid rolling-circle replication by binding to a triple 11-bp direct repeat (bind locus) and cleaving the DNA at a specific distant site located in a hairpin loop within the nic locus of the origin. The structure of native full-length RepB reveals a hexameric ring molecule, where each protomer has two domains. The origin-binding and catalytic domains show a three-layer α–β–α sandwich fold. The active site is positioned at one of the faces of the β-sheet and coordinates a Mn2+ ion at short distance from the essential nucleophilic Y99. The oligomerization domains (ODs), each consisting of four α-helices, together define a compact ring with a central channel, a feature found in ring helicases. The toroidal arrangement of RepB suggests that, similar to ring helicases, it encircles one of the DNA strands during replication to confer processivity to the replisome complex. The catalytic domains appear to be highly mobile with respect to ODs. This mobility may account for the adaptation of the protein to two distinct DNA recognition sites
Description47 p.-7 fig. Boer, Roeland et alt.
Publisher version (URL)http://dx.doi.org/10.1038/emboj.2009.125
URIhttp://hdl.handle.net/10261/53623
DOI10.1038/emboj.2009.125
ISSN0261-4189
E-ISSN1460-2075
Appears in Collections:(CIB) Artículos
(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
EMBO J._G. del Solar_2009.pdf4,42 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.