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Title

Virgin olive oil polyphenol hydroxytyrosol acetate inhibits in vitro platelet aggregation in human whole blood: Comparison with hydroxytyrosol and acetylsalicylic acid

AuthorsGonzález Correa, José A.; López-Villodres, Juan A.; Asensi, Rocío; Espartero, José L.; Rodríguez-Gutiérrez, Guillermo ; Cruz, José P. de la
KeywordsHydroxytyrosol acetate
Olive oil polyphenols
Platelet aggregation
Prostanoids
Nitric oxide
Issue DateApr-2009
PublisherCambridge University Press
CitationBritish Journal of Nutrition 101(8): 1157-1164 (2009)
AbstractHydroxytyrosol acetate (HT-AC) is a polyphenol present in virgin olive oil (VOO) at a proportion similar to hydroxytyrosol (HT) (160-479 μmol/kg oil). The present study was designed to measure the in vitro platelet antiaggregating activity of HT-AC in human whole blood, and compare this effect with that of HT and acetylsalicylic acid (ASA). The experiments were designed according to the standard procedure to investigate the activity of ASA. HT-AC and HT inhibited platelet aggregation induced by ADP, collagen or arachidonic acid in both whole blood and platelet-rich plasma (PRP). ASA and HT-AC had a greater effect in whole blood than in PRP when ADP or collagen was used as inducer. ASA and HT-AC had a greater effect in PRP+leucocytes than in PRP alone. All three compounds inhibited platelet thromboxane B2 and leucocyte 6-keto-prostaglandin F1α (6-keto-PF1α) production. The thromboxane/ 6-keto-PGF1α inhibition ratio (as an indirect index of the prostanoid equilibrium) was 10.8 (SE1) for HT-AC, 1.0 (SE 0.1) for HT and 3.3 (SE 0.2) for ASA. All three compounds stimulated nitric oxide production, although HT was a weaker effect. In our experiments only concentrations higher than 500 μM (HT) or 1 mM (HT-AC and ASA) inhibited 3-nitrotyrosine production. All three compounds inhibited the production of TNFα by leucocytes, with no significant differences between them. In quantitative terms HT-AC showed a greater antiplatelet aggregating activity than HT and a similar activity to that of ASA. This effect involved a decrease in platelet thromboxane synthesis and an increase in leucocyte nitric oxide production.
URIhttp://hdl.handle.net/10261/53609
DOI10.1017/S0007114508061539
Identifiersdoi: 10.1017/S0007114508061539
issn: 0007-1145
e-issn: 1475-2662
Appears in Collections:(IG) Artículos
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