English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/5354
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Mitochondrial Localization of the Mevalonate Pathway Enzyme 3-Hydroxy-3-methyl-glutaryl-CoA Reductase in the Trypanosomatidae

AutorPeña Díaz, Javier; Montalvetti, Andrea; Flores, Carmen-Lisset ; Constán Gutiérrez, Aurora Isabel; Hurtado Guerrero, Ramón; Souza, Wanderley de; Gancedo, Carlos ; Ruiz Pérez, Luis M.; González-Pacanowska, D.
Fecha de publicaciónmar-2004
EditorAmerican Society for Cell Biology
CitaciónMol Biol Cell. 2004 March; 15(3): 1356–1363
Resumen3-Hydroxy-3-methyl-glutaryl-CoA reductase (HMGR) is a key enzyme in the sterol biosynthesis pathway, but its subcellular distribution in the Trypanosomatidae family is somewhat controversial. Trypanosoma cruzi and Leishmania HMGRs are closely related in their catalytic domains to bacterial and eukaryotic enzymes described but lack an amino-terminal domain responsible for the attachment to the endoplasmic reticulum. In the present study, digitonin-titration experiments together with immunoelectron microscopy were used to establish the intracellular localization of HMGR in these pathogens. Results obtained with wild-type cells and transfectants overexpressing the enzyme established that HMGR in both T. cruzi and Leishmania major is localized primarily in the mitochondrion and that elimination of the mitochondrial targeting sequence in Leishmania leads to protein accumulation in the cytosolic compartment. Furthermore, T. cruzi HMGR is efficiently targeted to the mitochondrion in yeast cells. Thus, when the gene encoding T. cruzi HMGR was expressed in a hmg1 hmg2 mutant of Saccharomyces cerevisiae, the mevalonate auxotrophy of mutant cells was relieved, and immunoelectron analysis showed that the parasite enzyme exhibits a mitochondrial localization, suggesting a conservation between the targeting signals of both organisms.
DescripciónCopyright © by American Society for Cell Biology.-- Final full-text version of the paper available at: http://www.molbiolcell.org/content/vol15/issue3/
URIhttp://hdl.handle.net/10261/5354
DOI10.1091/mbc.E03-10-0720
ISSN1059-1524
Aparece en las colecciones: (IIBM) Artículos
(IPBLN) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
mevalonate.pdf292,02 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.