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Determination of 3,4-dihydroxyphenylglycol, hydroxytyrosol and tyrosol purified from olive oil by-products with HPLC in animal plasma and tissues

AuthorsRodríguez-Gutiérrez, Guillermo ; Wood, Sharon; Fernández-Bolaños Guzmán, Juan ; Duthie, Garry G.; Roos, Baukje de
KeywordsOlive oil
Animal tissues
Animal plasma
Issue Date15-Jun-2011
CitationFood Chemistry 126(4): 1948-1952 (2011)
AbstractA simple, fast and reliable method to quantify, simultaneously, 3,4-dihydroxyphenylglycol (DHPG), hydroxytyrosol (HT) and tyrosol (Ty) extracted and purified successfully from olive oil by-product, called alperujo, in animal plasma and tissues samples has been developed using a high-performance liquid chromatographic (HPLC) method with UV-Vis detection. Extraction of compounds is based on solid-phase extraction for plasma and homogenisation with zirconia beads and centrifugation for tissues. Calibration curves were linear for all three phenols at a relatively low concentration range (0.05-50 μg/mL). This method has acceptable accuracy (91-95% in plasma and 63-100% in tissues), precision (1.11-8.26% intra-day and 0.32-9.5% inter-day) and sensitivity for detecting low concentrations of these phenols in small plasma volumes and several animal tissues such as liver, heart, kidney, muscle, testes, white adipose tissue (WAT) and brain. © 2010 Elsevier Ltd. All rights reserved.
Identifiersdoi: 10.1016/j.foodchem.2010.12.044
issn: 0308-8146
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