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Protection from Staphylococcus aureus mastitis associated with poly-N-acetyl beta-1,6 glucosamine specific antibody production using biofilm-embedded bacteria

AuthorsPérez, Marta M.; Prenafeta, Antoni; Valle, Jaione; Penadés, José R. ; Rota, C.; Solano, Cristina; Marco, Juan Carlos; Grilló, María Jesús ; Lasa, Iñigo; Irache, Juan Manuel; Maira-Litran, Tomás; Jiménez-Barbero, Jesús ; Costa, Llorenç; Pier, Gerald B.; Andrés, Damián F. de ; Amorena, Beatriz
KeywordsS. aureus
poly-N-acetyl β-1,6 glucosamine
Issue Date14-Apr-2009
CitationVaccine 27(17):2379-2386(2009)
AbstractStaphylococcus aureus vaccines based on bacterins surrounded by slime, surface polysaccharides coupled to protein carriers and polysaccharides embedded in liposomes administered together with non-biofilm bacterins confer protection against mastitis. However, it remains unknown whether protective antibodies are directed to slime-associated known exopolysaccharides and could be produced in the absence of bacterin immunizations. Here, a sheep mastitis vaccination study was carried out using bacterins, crude bacterial extracts or a purified exopolysaccharide from biofilm bacteria delivered in different vehicles. This polysaccharide reacted specifically with antibodies to poly-N-acetyl-β-1,6-glucosamine (PNAG) and not with antibodies to other capsular antigens or bacterial components. Following intra-mammary challenge with biofilm-producing bacteria, antibody production against the polysaccharide, milk bacterial counts and mastitis lesions were determined. Bacterins from strong biofilm-producing bacteria triggered the highest production of antibodies to PNAG and conferred the highest protection against infection and mastitis, compared with weak biofilm-producing bacteria and non-cellular inocula. Thus, bacterins from strong biofilm bacteria, rather than purified polysaccharide, are proposed as a cost-efficient vaccination against S. aureus ruminant mastitis
Description8 páginas, 4 figuras, 4 tablas -- PAGS nros. 2386-2379
Publisher version (URL)http://dx.doi.org/10.1016/j.vaccine.2009.02.005
Appears in Collections:(CIB) Artículos
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