Please use this identifier to cite or link to this item:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

A high-fat meal promotes lipid-load and apolipoprotein B-48 receptor transcriptional activity in circulating monocytes

AuthorsVarela, Lourdes; Ortega, Almudena CSIC ORCID; Bermúdez, Beatriz CSIC ORCID; López, Sergio; Pacheco, Yolanda M. CSIC ORCID; Villar Ortiz, José CSIC; Abia, Rocío CSIC ORCID ; Muriana, Francisco J. G. CSIC ORCID
Issue DateMay-2011
PublisherAmerican Society for Nutrition
CitationAmerican Journal of Clinical Nutrition 93(5): 918-925 (2011)
AbstractBackground: The postprandial metabolism of dietary fats results in the production of apolipoprotein B-48 (apoB48)-containing triglyceriderich lipoproteins (TRLs), which cause rapid receptor-mediated macrophage lipid engorgement via the apoB48 cell surface receptor (apoB48R). Monocytes circulate together with apoB48-containing TRLs in the postprandial bloodstream and may start accumulating lipids even before their migration to tissues and differentiation to macrophages. Objective: We sought to determine whether circulating monocytes are equipped with apoB48R and whether, in the postprandial state, circulating monocytes accumulate lipids and modulate apoB48R transcriptional activity after intake of a high-fat meal. Design: In a crossover design, we studied the effect of a high-fat meal on fasting and postprandial concentrations of triglycerides, free fatty acids, cholesterol, and insulin in 12 healthy men. TRLs and monocytes were freshly isolated at fasting, hourly until the postprandial peak, and at the late postprandial phase. TRLs were subjected to triglycerides, apoB48, and apolipoprotein B-100 analyses; and lipid accumulation and apoB48R mRNA expression levels were measured in monocytes. Results: Monocytes showed a time-dependent lipid accumulation in response to the high-fat meal, which was paralleled by an increase in apoB48R mRNA expression levels. These effects were coincident only with an increase in apoB48-containing TRLs in the postprandial phase and were also observed ex vivo in freshly isolated monocytes incubated with apoB48-containing TRLs. Conclusion: In a setting of abundant plasma apoB48-containing TRLs, these findings highlight the role of dietary fat in inducing lipid accumulation and apoB48R gene transcription in circulating monocytes. © 2011 American Society for Nutrition.
Identifiersdoi: 10.3945/ajcn.110.007765
issn: 0002-9165
e-issn: 1938-3207
Appears in Collections:(IG) Artículos
(IBIS) Artículos

Show full item record
Review this work


checked on Jan 20, 2022


checked on Jan 22, 2022

Page view(s)

checked on Jan 27, 2022

Google ScholarTM




WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.