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Title

Activin A induces a non-fibrotic phenotype in smooth muscle cells in contrast to TGF-β

AuthorsGroenendijk, Bianca C. W.; Benus, Germaine F. J. D.; Klous, Anita; Pacheco, Yolanda M. ; Volger, Oscar L.; Fledderus, Joost O.; Ferreira, Valerie; Engelse, Marten A.; Pannekoek, Hans; Dijke, Peter ten; Horrevoets, Anton J. G.; Vries, Carlie J. M. de
KeywordsActivin A
Transforming growth factor-β (TGF-β)
Vascular smooth muscle cells
Smad1
Smad2
Extracellular matrix
Issue Date15-Jan-2011
PublisherElsevier
CitationExperimental Cell Research 317(2): 131-142 (2011)
AbstractAims: Activin A and transforming growth factor-β1 (TGF-β1) belong to the same family of growth and differentiation factors that modulate vascular lesion formation in distinct ways, which we wish to understand mechanistically. Methods and results: We investigated the expression of cell-surface receptors and activation of Smads in human vascular smooth muscle cells (SMCs) and demonstrated that activin receptor-like kinase-1 (ALK-1), ALK-4, ALK-5 and endoglin are expressed in human SMCs. As expected, TGF-β1 activates Smad1 and Smad2 in these cells. Interestingly, activin A also induces phosphorylation of both Smads, which has not been reported for Smad1 before. Transcriptome analyses of activin A and TGF-β1 treated SMCs with subsequent Gene-Set Enrichment Analyses revealed that many downstream gene networks are induced by both factors. However, the effect of activin A on expression kinetics of individual genes is less pronounced than for TGF-β1, which is explained by a more rapid dephosphorylation of Smads and p38-MAPK in response to activin A. Substantial differences in expression of fibronectin, alpha-V integrin and total extracellular collagen synthesis were observed. Conclusions: Genome-wide mRNA expression analyses clarify the distinct modulation of vascular lesion formation by activin A and TGF-β1, most significantly because activin A is non-fibrotic. © 2010 Elsevier Inc.
URIhttp://hdl.handle.net/10261/53323
DOI10.1016/j.yexcr.2010.10.007
Identifiersdoi: 10.1016/j.yexcr.2010.10.007
issn: 0014-4827
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