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Title

A colorimetric method for determination of γ-glutamyl-S-ethenyl- cysteine in narbon vetch (Vicia narbonensis L.) seeds

AuthorsSánchez Vioque, Raúl; Rodríguez Conde, María Fernanda; Vioque, Javier ; Girón-Calle, Julio ; Santana Méridas, Omar; Mozos-Pascual, Marcelino de los; Izquierdo-Melero, M. E.; Alaíz Barragán, Manuel
KeywordsAntinutritional factor
Feed
γ-Glutamyl-S-ethenyl-cysteine
Lodoplatinate
Legume
Sulfide
Issue Date15-Nov-2011
PublisherElsevier
CitationAnalytical Biochemistry 418: 180-183 (2011)
AbstractA new colorimetric method based on the bleaching of the iodoplatinate ion has been developed for fast and easy determination of γ-glutamyl-S- ethenyl-cysteine (GEC) in narbon vetch (Vicia narbonensis L.) seeds. The calibration curve showed a good correlation (r2 = 0.9959) between absorbance and GEC amounts from 5.5 to 33 μg (10-59.78 μmol/L). The limits of detection and quantification were 1.16 and 3.55 μmol/L, respectively, and no significant interferences from other sulfur-containing compounds were observed. The method showed excellent repeatability (relative standard deviation [RSD] = 0.28%), reproducibility (RSD = 4.4%), and accuracy (94%). Determination of GEC in 20 narbon vetch accessions yielded values that were in agreement with those reported previously using capillary electrophoresis and high-performance liquid chromatography methods. The method could be especially valuable for determination of GEC during the process of production of new low-GEC narbon vetch varieties. Copyright © 2011 Published by Elsevier Inc. All rights reserved.
URIhttp://hdl.handle.net/10261/53321
DOI10.1016/j.ab.2011.07.010
Identifiersdoi: 10.1016/j.ab.2011.07.010
issn: 0003-2697
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