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Characterization of exopolysaccharides produced by Bifidobacterium longum NB667 and Its cholate-resistant derivative strain IPLA B667dCo

AuthorsSalazar, Nuria CSIC ORCID; Ruas-Madiedo, Patricia CSIC ORCID ; Prieto, Alicia CSIC ORCID ; Calle Jiménez, Luis Pablo CSIC; González de los Reyes-Gavilán, Clara CSIC ORCID
Issue Date1-Feb-2012
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 60(4): 1028-1035 (2012)
AbstractBifidobacteria are natural members of the human intestinal microbiota and some strains are being used as probiotics. Adaptation to bile can allow them to increase survival in gastrointestinal conditions, thus improving their viability. Bifidobacterium longum NB667 and the cholate-resistant strain B. longum IPLA B667dCo produced exopolysaccharides (EPS) that were partially characterized. Analysis by size exclusion chromatography-multiangle laser light scattering indicated that the EPS crude fractions of both strains contained two polymer peaks of different molar mass. On the basis of chromatographic techniques both peaks appeared to be heteropolysaccharides. The smaller peak was mainly composed of glucose, galactose and rhamnose whose molar ratios and linkage types showed slight variations between the EPS fractions of both strains. The bigger peak consisted of glucose and galactose; the monosaccharide composition was identical in the EPS fractions of the two microorganisms, but their infrared spectra presented some differences regarding compounds other than carbohydrates that seem to be associated to the polymer. Differences in the composition of EPS fractions did not affect the capability of crude EPS from B. longum to be fermented by the human intestinal microbiota in fecal batch cultures. © 2012 American Chemical Society.
Identifiersdoi: 10.1021/jf204034n
issn: 0021-8561
e-issn: 1520-5118
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