English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/53231
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Adaptive changes on the expression and functionality of CB1 receptors induced by neuropathic pain

AutorDíaz, Álvaro ; Rojo, María Luisa ; Rodríguez-Gaztelumendi, A. ; Pazos, Ángel
Fecha de publicación2010
EditorFederation of European Neuroscience Societies
Citación7th FENS Forum of European Neuroscience (2010)
ResumenNeuropathic pain is a debilitating form of chronic pain resulting from nerve injury and it is often refractory to conventional pharmacotherapies. Preclinical and clinical studies have demonstrated that cannabinoids provide a potential approach to pain management with a novel therapeutic target and mechanism. In this study we have analyzed, by autoradiographic methods, the expression and the functionality of spinal and supraspinal CB1 receptors in a rat model of neuropathic pain: the spared nerve injury (SNI). All the autoradiographic studies were performed 14 days postsurgery after confirming the presence of mechanical hyperalgesia and allodynia. CB1 receptor density was determined using CP55, 940 (3 nM) and CB1 receptor functionality by measuring the stimulation of [35S]GTPgammaS binding induced by the cannabinoid agonist WIN 55, 212-2 (10 ¿M). Autoradiographic density of CB1 receptor was found to be significantly decreased (vs. sham-operated animals) in cingulate cortex (-31%), lateroventral septal nuclei (-27%), CA1-CA2 (-21%) and dentate gyrus of hippocampus (-20%), but increased in the median raphe nucleus (+86%). CB1 receptor-mediated stimulation of [35S]GTPgammaS binding was found to be significantly increased in septal nuclei (+44%), substantia nigra (+53%) and ventral parabraquial nuclei (+32%) but reduced in dentate gyrus of hippocampus (-17%) and median raphe nucleus (-61%). At the level of the dorsal horn of the lumbar spinal cord, SNI induced a significant reduction in CB1 receptor density (-30% vs. sham-operated animals) but no significant change in CB1 functionality was observed. Our results demonstrate the existence of regional modifications in the expression and functionality of CB1 receptors following peripheral nerve injury. These adaptive changes on CB1 receptor signaling may help to understand the pathogenesis of neuropathic pain and to elucidate the mechanism of action of cannabinoids as analgesics in this type of chronic pain.
DescripciónTrabajo presentado al: "7th FENS Forum of European Neuroscience" celebrado en Amsterdam del 3 al 7 de julio de 2010.
URIhttp://hdl.handle.net/10261/53231
Aparece en las colecciones: (IBBTEC) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.