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dc.contributor.authorSousa Silva, Marta-
dc.contributor.authorFortes, Ana M.-
dc.contributor.authorTestillano, P. S.-
dc.contributor.authorRisueño, María Carmen-
dc.contributor.authorSuares, María Salomé-
dc.identifier.citationEuropean Journal of Cell Biology 83(8):425-433(2004)es_ES
dc.description9 páginas, 5 figuras -- PAGS nros. 425-433es_ES
dc.description.abstractThe expression and subcellular localization of extracellular signal-regulated kinase 1 or 2 (ERK1/2) homologues (HLERK1/2) during the process of organogenic nodule formation in Humulus lupulus var. Nugget was studied using antibodies specific for ERK1 and ERK2, and for phosphorylated mitogen-activated protein kinases (MAPKs). The increase in HLERK levels, detected by Western blotting 12 hours after wounding suggests their involvement in response to the wounding treatment applied for morphogenesis induction. In dividing cambial cells, occurring in between 4 and 7 days after morphogenesis induction, as well as in dividing prenodular cells (15 days after induction) HLERK1 and/or 2 were localized in the nucleus. However, as soon as nodular cells start proliferating to form shoot meristems, HLERK1 and 2 were detected in the cytoplasm and not in the nucleus. The data reported account for a differential expression and activation of HLERK1 and HLERK2 throughout the process of nodule formation and plantlet regeneration. HLERK1 appears to be expressed in the stages of nodule formation and plantlet regeneration, playing a possible role in controlling cell proliferation and differentiation. HLERK2 may be induced as a response to reactive oxygen species (ROS) generated by wounding of internodes as its expression is reduced in liquid medium with less oxygen availability compared to solid medium. However, addition of a ROS inhibitor to the liquid medium does not result in a further decrease in the HLERK2 leveles_ES
dc.description.sponsorshipFunding for this work was provided by the Portuguese Foundation for Science and Technology through a PhD grant to M. S. Silva (PRAXIS XXI /BD/13730/97). This work was also supported by the Portuguese Project PCTI/1999/BME/32734, by the Spanish Projects DGESIC PB 98 ± 0678, MCyT B052002 ± 03572 and CAM 076/0054/2000, and by the Portuguese-Spanish Joint Project Accio¬ n Integrada HP2000 ± 0065es_ES
dc.subjectHumulus lupulus var. Nuggetes_ES
dc.subjectNodule formationes_ES
dc.titleDifferential expression and cellular localization of ERKs during organogenic nodule formation from internodes of Humulus lupulus var. Nuggetes_ES
dc.description.peerreviewedPeer reviewedes_ES
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