English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/53135
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Expresion of LOX during organogenic nodule formation from hoop internodes (Humulus lupulus var. Nugget)

AuthorsFortes, Ana M.; Coronado, María José ; Testillano, P.S. ; Risueño, María Carmen ; Pais, María Salomé
KeywordsHumulus lupulus var. Nugget
immunolocalization
lipid peroxides
lipoxygenases
morphogenesis
Issue DateFeb-2004
PublisherHistochemical Society
CitationJournal of Histochemistry & Cytochemistry 52(2):227-241(2004)
AbstractStudy of lipoxygenase expression (LOX; EC 1.13.11.12) during organogenic nodule formation in hop (Humulus lupulus var. Nugget) showed that LOXs are developmentally regulated throughout the process, suggesting their involvement in the response of internodes to wounding, nodule formation, and plantlet regeneration from these nodules. LOX activity and lipid peroxides exhibited a huge increase during the first week of culture, which may indicate a role for LOX and LOX products in response to wounding in hop, as reported for other systems. Western blotting analysis showed a de novo synthesis of LOX isoenzymes in response to wounding and the detection of three different isoenzymes. Confocal analysis of LOX immunofluorescence revealed the presence of the enzyme in cortical cells of induced internodes and in prenodular cells, mostly appearing as cytoplasmic spots. Some of them were identified as lipid bodies by cytochemical and double immunofluorescence assays, suggesting the involvement of a lipid body LOX during nodule formation. Immunogold labeling detected LOX in peroxisomes, lipid bodies, and plastids of nodular cells. Quantification of the labeling density provided statistical significance for the localization of LOX (three different isoenzymes) in the three compartments, which suggested a possible involvement of LOX in metabolic functions of these organelles during organogenic nodule formation and plantlet regeneration
Description15 páginas, 10 figuras -- PAGS nros. 227-241
Publisher version (URL)http://dx.doi.org/10.1177/002215540405200211
URIhttp://hdl.handle.net/10261/53135
DOI10.1177/002215540405200211
ISSN0022-1554
E-ISSN1551-5044
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.