English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/53111
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis

AuthorsTestillano, P.S. ; Georgiev, Sevdalin; Mogensen, H. Lloyd; Coronado, María José ; Dumas, Christian; Risueño, María Carmen ; Matthys-Rochon, Elisabeth
Issue Date4-May-2004
PublisherSpringer
CitationChromosoma 112(7):342-349(2004)
AbstractA multidisciplinary study was carried out to analyse the chromosome doubling process during the early stages of in vitro maize microspore embryogenesis. The main stages (microspore derivatives) that were formed in the course of the culture were analysed. Chromosome number was determined from squashed cells, and DNA content was measured by cytometry. In parallel, an ultrastructural analysis of the microspore derivatives demonstrated the occurrence of a nuclear fusion process. It seems likely that nuclear fusion ensures chromosome doubling at early stages of induced microspore embryogenesis. It occurs precisely at the 5/7 day stage in the embryonic domain and probably leads to polyploidy in the endosperm domain of the microspore derivatives. As a conclusion a scheme summarises the results and proposes an interpretation of the sequence of chromosome doubling events during early maize microspore embryogenesis. Understanding of this process will be important for future efforts to increase the percentage of homozygous plants for crop improvement
Description8 páginas, 5 figuras -- PAGS nros. 342-349
Publisher version (URL)http://dx.doi.org/10.1007/s00412-004-0279-3
URIhttp://hdl.handle.net/10261/53111
DOI10.1007/s00412-004-0279-3
ISSN0009-5915
E-ISSN1432-0886
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.