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dc.contributor.authorSeguí-Simarro, José María-
dc.contributor.authorTestillano, P.S.-
dc.contributor.authorJouannic, Stefan-
dc.contributor.authorHenry, Yves-
dc.contributor.authorRisueño, María Carmen-
dc.identifier.citationHistochemistry and Cell Biology 123(4-5):541-551(2005)es_ES
dc.description11 páginas, 7 figuras -- PAGS nros. 541-551es_ES
dc.description.abstractPlant mitogen-activated protein kinase (MAPK) cascades are involved in extracellular stress signalling pathways, leading to different cellular responses. Stress-induced microspore embryogenesis involves the internalization of an extracellular stress signal, generating a number of cellular responses where MAPK cascades might be involved. These responses include a change of the developmental programme, the entry into an early proliferative stage and, subsequently, into differentiation stages during haploid embryogenesis. In this work we studied the expression during microspore embryogenesis of several kinases, to assess their putative role in these events. The known Brassica napus MAP kinase kinase kinases (MAP3Ks BnMAP3K1, BnMAP3K1 and BnMAP3K, the BnBSK kinase and B. napus extracellular signal-regulated kinase (ERK) homologues were analysed by electron microscope (EM) in situ hybridization, immuno-gold labelling, immunofluorescence and western blotting. The differential in situ expression of these kinases suggests a role for them during embryogenesis. Two different expression patterns were observed, indicating a different regulation. BnMAP3K1, BnMAP3K, and the ERKs showed a pattern consistent with a role mainly in proliferative events. Conversely, BnMAP3K1 and BnBSK, presented a pattern that suggested an involvement in differentiation stages. In addition, ERK homologues migrate to the nucleus immediately after induction, being found in a phosphorylated state in a larger amountes_ES
dc.description.sponsorshipThis work was supported by research projects funded by EU BIO4-CT-0275, Spanish DGESIC PB98-0678, MCyT BOS2002-03572, and CM 07G/0026/2003es_ES
dc.subjectBrassica napuses_ES
dc.subjectHigh resolution in situ hybridizationes_ES
dc.subjectMAP kinase cascadeses_ES
dc.subjectMicrospore embryogenesises_ES
dc.titleMitogen-activated protein kinases are developmentally regulated during stress-induced microspore embryogenesis in Brassica napus Les_ES
dc.description.peerreviewedPeer reviewedes_ES
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