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Mitogen-activated protein kinases are developmentally regulated during stress-induced microspore embryogenesis in Brassica napus L

AuthorsSeguí-Simarro, José María ; Testillano, P. S. ; Jouannic, Stefan; Henry, Yves; Risueño, María Carmen
KeywordsBrassica napus
High resolution in situ hybridization
MAP kinase cascades
Microspore embryogenesis
Issue DateJun-2005
CitationHistochemistry and Cell Biology 123(4-5):541-551(2005)
AbstractPlant mitogen-activated protein kinase (MAPK) cascades are involved in extracellular stress signalling pathways, leading to different cellular responses. Stress-induced microspore embryogenesis involves the internalization of an extracellular stress signal, generating a number of cellular responses where MAPK cascades might be involved. These responses include a change of the developmental programme, the entry into an early proliferative stage and, subsequently, into differentiation stages during haploid embryogenesis. In this work we studied the expression during microspore embryogenesis of several kinases, to assess their putative role in these events. The known Brassica napus MAP kinase kinase kinases (MAP3Ks BnMAP3K1, BnMAP3K1 and BnMAP3K, the BnBSK kinase and B. napus extracellular signal-regulated kinase (ERK) homologues were analysed by electron microscope (EM) in situ hybridization, immuno-gold labelling, immunofluorescence and western blotting. The differential in situ expression of these kinases suggests a role for them during embryogenesis. Two different expression patterns were observed, indicating a different regulation. BnMAP3K1, BnMAP3K, and the ERKs showed a pattern consistent with a role mainly in proliferative events. Conversely, BnMAP3K1 and BnBSK, presented a pattern that suggested an involvement in differentiation stages. In addition, ERK homologues migrate to the nucleus immediately after induction, being found in a phosphorylated state in a larger amount
Description11 páginas, 7 figuras -- PAGS nros. 541-551
Publisher version (URL)http://dx.doi.org./10.1007/s00418-004-0749-y
Appears in Collections:(CIB) Artículos
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