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Stress and DNA assembly differences on cantilevers gold coated by resistive and e-beam evaporation techniques

AuthorsArroyo-Hernández, María ; Tamayo de Miguel, Francisco Javier ; Costa Krämer, José Luis
Issue Date2009
PublisherAmerican Chemical Society
CitationLangmuir : the ACS journal of surfaces and colloids 25: 10633-10638 (2009)
AbstractChanges in the sign of differential surface stress of gold-coated cantilevers produced by thiol-derivatized singlestranded DNA immobilization are observed, depending on the method used to deposit the gold. While the DNA immobilization on e-beam gold-coated cantilevers produces a compressive differential surface stress in the metallic layer, the opposite is observed for resistively coated cantilevers under the same immobilization conditions. The gold films exhibit quite a similar morphology, and the immobilization differences seem to be related to the charge state of the metallic layer surface. This in turn produces a different distribution of the orientation of the DNA strands on the gold layer. A tentative explanation for the observed effect is proposed. © 2009 American Chemical Society.
Identifiersdoi: 10.1021/la900696f
issn: 0743-7463
Appears in Collections:(IMN-CNM) Artículos
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