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Título

Toll-like receptor stimulation differentially regulates vasoactive intestinal peptide type 2 receptor in macrophages

AutorHerrera, Juan Luis; González-Rey, Elena; Fernández Montesinos, Rafael ; Quintana, Francisco J.; Najamanovich, Rafael; Pozo, David
Palabras claveVIP
Neuroimmunology
Neuropeptides
Inflammation
Toll-like receptors
Fecha de publicaciónsep-2009
EditorJohn Wiley & Sons
CitaciónJournal of Cellular and Molecular Medicine 13(9b): 3209-3217 (2009)
ResumenVasoactive intestinal peptide (VIP) was originally isolated as a vasodilator intestinal peptide, then as a neuropeptide. In the immune system, VIP is described as an endogenous macrophage-deactivating factor. VIP exerts its immunological actions in a paracrine and/or autocrine manner, through specific receptors. However, very little is known about the molecular regulation of VIP type 2 receptor (VPAC(2)) in the immune system. We now report that different toll-like receptor (TLR) ligands selectively regulate the VPAC(2) receptor gene and show a gene repression system controlled by key protein kinase signalling cascades in macrophages. VPAC(2) gene expression is regulated by gram-positive (TLR2 ligands) and gram-negative bacteria wall constituents (TLR4 ligands). Moreover, VPAC(2) is tightly regulated: TLR2- or TLR2/6- but not TLR2/1-mediated mechanisms are responsible for the induction of VPAC(2). TLR stimulation by viral or bacterial nucleic acids did not modify the VPAC(2) mRNA levels. Remarkably, imiquimod--a synthetic TLR7 ligand--led to a potent up-regulation of VPAC(2) gene expression. TLR5 stimulation by flagellin present in gram-positive and gram-negative bacteria did not affect VPAC(2) mRNA. The p38 mitogen-activated protein kinase (MAPK) activity accounted for the TLR4-mediated induction of VPAC(2) gene expression. Surprisingly, our data strongly suggest for the first time a tightly repressed control of VPAC(2) mRNA induction by elements downstream of MAPK kinase 1/2, PI3K/Akt, and particularly Jun-NH(2)-terminal kinase signalling pathways.
URIhttp://hdl.handle.net/10261/52545
DOI10.1111/j.1582-4934.2009.00662.x
Identificadoresdoi: 10.1111/j.1582-4934.2009.00662.x
issn: 1582-1838
ReferenciasPMID: 20196778
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