English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/52506
Título

Stimulation of direct-repeat recombination by RNA polymerase III transcription

AutorDíaz de la Loza, M. Carmen ; Wellinger, Ralf Erik ; Aguilera, Andrés
Palabras clavetRNASUP53
Transcription-associated recombination
4-NQO
rrm3Δ
RFB
Fecha de publicación1-may-2009
EditorElsevier
CitaciónDNA Repair 8(5): 620-626 (2009)
ResumenEukaryotic cells have to regulate the progression and integrity of DNA replication forks through concomitantly transcribed genes. A transcription-dependent increase of recombination within protein-coding and ribosomal genes of eukaryotic cells is well documented. Here we addressed whether tRNA transcription and tRNA-dependent transcription-associated replication pausing leads to genetic instability. Thus, we designed a plasmid based, LEU2 direct-repeat containing system for the analysis of factors that contribute to tRNA(SUP53)-dependent genetic instability. We show that tRNA(SUP53) transcription is recombinogenic and that recombination can be further stimulated by deletion of the 5' to 3' helicase Rrm3. Furthermore, tRNA(SUP53)-dependent recombination was markedly increased in the presence of 4-NQO in rrm3Delta cells only. The frequency of recombination events mediated by tRNA(SUP53) transcription does not correlate with the appearance and intensity of replication fork pausing sites. Our results provide evidence that the convergent encounter of replication and RNA polymerase III transcription machineries stimulates recombination, although to a lesser extent than RNA polymerase I or II transcription. However, there is no correlation between recombination and the specific replication fork pausing sites found at the tRNA (SUP53) gene. Our results indicate that tRNA-specific replication fork pausing sites are poorly recombinogenic.
URIhttp://hdl.handle.net/10261/52506
DOI10.1016/j.dnarep.2008.12.010
Identificadoresdoi: 10.1016/j.dnarep.2008.12.010
issn: 1568-7864
ReferenciasPMID: 19168400
Aparece en las colecciones: (CABIMER) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.